By Carlos C Perez-Marin

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A small abdominal incision is made, the uterus exteriorized and the semen injected directly into the uterine lumen through the uterine wall. Disadvantages of this procedure are the relatively high costs as well as the anesthetic and surgical risk. Surgical insemination is considered an unethical procedure in some countries (Linde-Forsberg, 1991). Insemination doses are usually composed of 100-150 x 106 motile spermatozoa. , 2006). 2 AI techniques in horses AI is a widely practiced breeding method in most sport horse breeds worldwide.

Semen is deposited intravaginally. Due to the fact that rabbits have two separate uterine horns and cervices, intracervical AI is not performed as it would be required to release semen into both cervices. Other peculiarities regarding AI in rabbits are that females should be kept separate from males for about 19 days before breeding to exclude pseudopregnancy, that timing of AI is based on general behaviour and vulva colour of the doe, and that rabbits are induced ovulators that always require induction of ovulation when AI is used.

Garner, D. , Gledhill, B. , & Van Dilla, M. A. (1983). Quantification of the X- and Y-chromosome bearing spermatozoa of domestic animals by flow cytometry. Biology of Reproduction, 28, 312-321. Garner, D. , & Seidel Jr, G. E. (2008). History of commercializing sexed semen for cattle. Theriogenology, 69(7), 886-895. , & Kubisch, H. M. (2009). Postmortem recovery and cryopreservation of spermatozoa from the vas deferens of rhesus macaques (Macaca mulatta). Theriogenology, 72(6), 834-840. Goovaerts, I.

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